Audio can be sourced via embedded audio, the capture device's audio inputs, through a separate audio interface, or even be played from a pre-recorded audio file.
Audio Mixer: Adjust audio levels and add audio effects to any source. There is a built-in audio mixer so you won't have to rely on a separate hardware mixer. Integrates with Facebook, Twitch and YouTube. Up to 4K resolution is supported, depending on the limitations of the card and computer hardware. Select a driver from the dropdown menu and then choose from any plug-in associated with that driver from the next drop down and hit OK. Now click on the + button to add a plug-in. Settings Audio Outputs allows up to eight independently controllable audio outputs (excluding Headphones output) If both Master Output and Headphones are set to the same device, Headphones will take priority. Use a video I/O card such as a Blackmagic or AJA product to capture from a live camera or external video feed. Go to the audio mixer and click on the gear icon on either the master or an individual input to open the Audio Settings Plugins tab. An average workstation configuration with dedicated GPU can support about eight HD (or two 4K) sources.
In practice, the total number will depend on the limitations of your computer, GPU, and capture hardware. VMix 4K Live Production Streaming Mixing Software supports up to 1000 sources, including virtual as well as hardware sources.